Currently the monitoring regarding the emergence of antimicrobial-resistant Campylobacter spp. derived from humans is undertaken as research activities by the Tokyo Metropolitan Institute of Public Health, as part of the food safety assurance and promotion research project, with Grants for research from the Ministry of Health, Labour and Welfare of Japan.
Antimicrobial susceptibility tests were conducted by the disk method, in accordance with the CLSI standards in US. The 116 C. jejuni strains and 8 C. coli strains that were isolated from the stool of diarrhea cases at hospitals in Tokyo in 2015 were tested using imicrobials such as tetracycline (TC), nalidixic acid (NA), ciprofloxacin (CPFX), norfloxacin (NFLX), ofloxacin (OFLX), and erythromycin (EM).
To identify the emergence of antimicrobial-resistant C. jejuni /C. coli on a wide-area basis, it is required to standardize tested antimicrobials, implementation methods, assessment criteria, and other details. However, no standardized methods have been indicated regarding antimicrobial susceptibility tests for Campylobacterspp. It is required to conduct antimicrobial susceptibility tests using common methods not only for strains isolated from humans, but also for strains isolated from food and food-producing animal, in order to know the emergence of antimicrobial- resistant bacteria nationwide.