Tables show the total use of antimicrobials in humans, food producing animals, aquatic animals, companion animals, antimicrobial feed additives, and agrochemicals. Antimicrobial selection pressure in Japan from a One Health perspective is highest among tetracyclines at 19-21%, followed by penicillins at 13-15%, and macrolides at 11-13%. Use of both penicillins and macrolides has been growing over recent years, so caution regarding future trends will be required. On the other hand, the fact that barely any changes in cephalosporins and fluoroquinolones were observed is attributed to differences in the antimicrobials that can be used in humans and in non-humans. Cephalosporins and fluoroquinolones are not used as drugs for aquatic animals or as antimicrobial feed additives or agrochemicals. As third-generation cephalosporins and fluoroquinolones are critically important antimicrobials for human medicine, they are positioned as a second-line drug for food-producing animals and used with great caution, based on assessments by the Food Safety Commission of Japan of their impact on human health via food. Accordingly, they account for only a small proportion of antimicrobials used in animals. Third-generation cephalosporins and fluoroquinolones also are positioned as second-line drugs for companion animals and are consequently used with caution.